A microplate method for functional complement assay
نویسندگان
چکیده
منابع مشابه
Fluorometric method for phenylalanine microplate assay adapted for phenylketonuria screening.
We adapted the method of McCaman and Robins for fluorometry of phenylalanine to a microplate assay for routine phenylketonuria screening. Sensitivity is 15 mumol/L for the plasma assay and 30 mumol/L for the dried blood-spot assay, with CV less than 10% for both assays. Results for human plasma by microplate assay correlated well (r = 0.99) with results of amino acid analyzer determination of p...
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A fluorometric assay in microtitre plates for the screening of phenylketonuria was evaluated and adapted to a neonatal screening programme. Using this assay, it is possible to determine quantitatively the phenylalanine concentration in dried blood spots on filter paper. The test exhibited a linear calibration curve with a good slope as well as sufficient precision and accuracy in the statistica...
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ژورنال
عنوان ژورنال: Japanese Journal of Clinical Immunology
سال: 1983
ISSN: 0911-4300,1349-7413
DOI: 10.2177/jsci.6.167